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EMS GloQube® Plus & Accessories - Glow Discharge System for TEM Grids
Product Details
Description
Rapid, reliable results with the NEW EMS GloQube® Plus
Unique Dual Chamber Processing, Safe Handling of Reagents
The GloQube® Plus has two independent vacuum chambers:
- Clean chamber, designed for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas
- Vapor chamber, designed for use with reagents such as methanol and alkylamine. With operator safety firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is convenient and reliable – the vial, located in its holder, is inserted into a shielded needle using a simple bayonet fitting.
To prevent accidental damage, the high voltage lead is shielded. The plasma current is variable by adjustment of the vacuum level using an argon leak valve with the plasma voltage being preset. For maximum sputter coating efficiency, the gas injector system ensures that argon gas enters the chamber close to the plasma discharge. Venting is to argon.
Key Features
- Automatic control of vapor and air introduction
- Flush and purge cycles of vapor chamber and gas line
- Two chambers for separate in-air and in-vapor processes without contamination*
- Negative and positive discharge modes
- Safe vapor delivery using septum-sealed vials
- Automatic valving between chambers to prevent cross-contamination
- Fast and/or soft venting options
- Single door for easy sample loading
- Adjustable three height sample stage
- Intuitive touch screen control
- Loaded with typical standard recipes
- Password protected user profiles and programmable user recipes
The primary application of the EMS GloQube® is the hydrophilization (wetting) of carbon-coated TEM support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.
Glow Discharge Process
| Surface State | Charge | Atmosphere | Typical applications |
| Hydrophilic | Negative | Air | Hydrophilization and cleaning of carbon coated TEM grids |
| Hydrophilic | Positive | Air* – with magnesium acetate post-treatment | Nucleic acid adhesion to carbon films |
| Hydrophobic** | Positive | Alkylamine | Controlled orientation and improved adhesion of negatively charged proteins, antibodies and nucleic acids |
| Hydrophobic** | Negative | Methanol | Controlled orientation and improved adhesion of positively charged protein molecules (e.g. ferritin, cytochrome c) |
* Air followed by post-treament with magnesium acetate by the user.
** Hydrophobic, as noted above, may represent a hydrophilic sample of less than 90 degree contact angle.
Unique Dual Chamber Processing, Safe Handling of Reagents
The GloQube® has two independent vacuum chambers:
- Clean chamber, designed for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas
- Vapor chamber, designed for use with reagents such as methanol and alkylamine. With operator safety firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is convenient and reliable – the vial, located in its holder, is inserted into a shielded needle using a simple bayonet fitting.
To prevent accidental damage, the high voltage lead is shielded. The plasma current is variable by adjustment of the vacuum level using an argon leak valve with the plasma voltage being preset. For maximum sputter coating efficiency, the gas injector system ensures that argon gas enters the chamber close to the plasma discharge. Venting is to argon.
Easy sample loading, fast turnaround times
Each chamber can accommodate two 25 x 75 mm glass microscopes slides. Loading could not be easier using draw-style chamber doors and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. For additional convenience – and to allow easy access for chamber cleaning – the stages can be completely removed.
Touch screen control – rapid data input, simple operation
The intuitive touch screen allows multiple users to rapidly input and store preferred process "recipes". Typical default glow discharge protocols are loaded as standard. Additionally, help files and useful maintenance data such as system on time and time since last clean are readily available to the operator. An Ethernet communications port is included for software updates.
Vacuum, automatic valving and controlled venting
The GloQube® has automatic valving between chambers which maintains cleanliness by preventing cross-contamination. At the end of a process run, automatic soft venting to atmosphere through filtered inlets ensures TEM grids are not disturbed. The GloQube® requires a single vacuum pump working in the 0.1 to 1 mbar range. A typical pump time to operational vacuum is 60 seconds.
Accessory kit included consisting of the following:
750 mm long flexible stainless steel vacuum tube with clamps, fuses, glass vials, vial caps and sealing washers, needle (spare).
Specifications
Power and Processes
| Plasma current | 1-50 mA |
| HV power supply | 30W |
| Maximum voltage | 800V |
| Electrode polarity – clean chamber | DC glow positive DC glow negative |
| Electrode polarity – vapor chamber | DC glow positive DC glow negative |
| Sample stage | 125 x 100 mm with location for two glass slides 25 x 75 mm or a 36 place grid holder |
| Sample stage operational heights | Adjustable 12.5 mm (0.5"), 22.5 mm (0.9") or 35 mm (1.38") |
| Pump hold time requirement | 0-72 hours |
| Process time | 1-900 seconds |
Safety
| Chamber vent inlets | Filtered air inlets with slow vent to minimise sample disturbance |
| Onboard reagent storage | Reagents (e.g. methanol or alkylamine) are contained in reusable sealed glass vials to minimize exposure to hazards. |
| High voltage safety interlocks | Hardware safety interlocked and software for process control |
Vacuum
| Vacuum control | Intergrated pirani gauge |
| Working vacuum range | 0.1 to 1 mbar |
| Vacuum pump minimum requirements | 5 m3/hr, 3600 l/m, 0.03 mbar ultimate vacuum. Inlet flange: KF 16 |
| Pumping time | Typical pump time to an operational vacuum of 0.1 mbar in 60 seconds |
| Vacuum isolation | Isolation valves to switch vacuum and prevent process chamber cross-contamination |
User Interface
| User interface | Full graphical interface with touch screen buttons and controls. In addition to displaying profiles, parameters, help screen and maintenance information are available |
| Profiles and profile logging | Capability to store 100 user profiles (name, date, time, vacuum, current and polarity) |
Dimensions and Communications
| Chamber size | 100 mm W x 100 mm H x 127 mm D (3.94" x 3.94" x 5") |
| Instrument size | 336 mm H x 364 mm D (13.2" x 14.3") |
| Instrument weight | 19.5 kg (42.9 lbs) |
| Pump (optional) | 391 mm W x 127 mm D x 177 mm H (15.4" x 5" x 7") |
| Pump weight | 16 kg (35.3 lbs) |
| Footprint with optional pump | 366 mm W x 600 mm D x 336 mm H (14.4" x 23.6" x 13.2") |
| Power requirements | 120 V 60 Hz, 15 A or 230 V 50 Hz, 10 A |
| Instrument power rating | 100-240 V AC 60/50 Hz 700 VA including pump, IEC inlet |
| Optional pump power rating | 115/230 V 60/50 Hz 450 W |
| Communication port | USB interface |
More Information
| Application |
EM
SEM
TEM
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Starting at €199.90 €237.88
A unique preparation tray for TEM Grid staining and/or using in a coating device or evaporator. The trays have a M4 tapped hole for inserting a pin mount or a handle for manipulation. The trays are made of medical grade aluminium and are available in two sizes.
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