Fluoro-Gel with PPD, Mounting Medium

Fluoro-Gel Mounting Medium with a strong anti-fading agent 1,4-phenylenediamine is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula preserves rapid photobleaching of FITC, Texas Red, AMCA, Alexa Fluoro 388, Alexa Fluoro 594, Tetramethylrhodamine and Redox. The fluorescence is retained during prolong storage at 4°C in the dark. This medium contains Phenylenediamine and is not suitable for immunofluorescence of Cy dyes, Phycoerythrin (R-PR) phycocyanin (PC), and allophycocyanin (APC). Prepared from TRIS buffer. Ready-to-use mounting medium with dark coffee color, this is normal, and does not interfere with immunofluorescence.

Instruction for use

1. Bring the vial to room temperature.
2. Rinse slide to be mounted with DISTILLED OR DEIONIZED WATER, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface away from light.
3. Turn the vial upside down and open the dropper to remove any air bubbles.
4. Apply 2-3 drops of mounting medium directly on top of the specimen.
5. Let stand at room temperature for about 3-5 minutes in the dark.
6. Apply cover slip carefully avoiding air bubbles.
7. The specimen is ready for visualization under a microscope.
8. One can seal the edges of cover slip with nail polish, any organic medium. If a coverslip is not sealed air bubbles will appear in few days.
9. Method for applying Coverslip: Put 1-2 drops of Mounting medium on the specimen. After 3-4 minutes apply coverslip carefully avoiding air bubbles. Put Kimwipes on the top of coverslip, press gently to remove excess mounting medium. With 200 micropipette add organic mounting medium to seal the edges. Incubate at 37°C for one hour in the dark to dry organic mounting medium.
10. For storage it is recommended that the slide be stored in the dark at 2-8°C.

Removal of coverslip
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deionized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.

Warning
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
“In vitro laboratory products for research”