Array Tomography is one way to acquire 3D data series of ultra or semi-thin sections of a tissue sample. Thereby, the sample is cut with an ultramicrotome and the sections are collected on a substrate for subsequent imaging with a SEM. Depending on the research project more or less sections are required to completely reconstruct a region of interest.
In comparison to Serial Block-Face Scanning EM (SBFSEM) and Focused Ion Beam (FIB), Array Tomography is a non-destructive technique. The collected sections can be stored, re-imaged at difference regions and resolutions, re-stained with heavy metals to enhance contrast or with fluorescent markers for correlative light and electron microscopy (CLEM).