PHEM Buffer for Electron Microscopy and Immuncytochemistry

PHEM buffer (PIPES, HEPES, EGTA, MgSO4) is ideally suited for the preparation of tissue and cell cultures in immunocytochemical applications. Compared to PBS, it enables superior staining of intracellular antigens while having minimal impact on biochemical reactions and enzyme activity. Additionally, PHEM offers excellent preservation of cellular ultrastructure, especially microtubules, which is crucial for sample preparation for electron microscopy.