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Substitutes for Uranyl Acetate

Since many decades Uranyl Acetate is used as a main compound for post-staining of ultrathin sections of biological samples to generate contrast for electron microscopy. Usually, this post-staining step is followed by staining with Lead Citrate for contrast enhancement.
Uranyl Acetate and Uranyl Formate are further used for negative staining. Here, the staining agent creates an electron dense coating surrounding the sample enabling TEM imaging. Negative staining is mainly used for TEM visualization of small particles such as macromolecules, extracted cellular organelles, viral particles or other small biological structures.
The radioactivity of Uranyl compounds and the associated legal restrictions were the driving force for the development of new non-radioactive substitutes. Depending on the sample and structures to examine the substitutes show similar staining results.

UranyLess & Lead Citrate

For ultra-thin sections, you won´t do anything wrong with these two bodies.
Uranyless EM Stain

Starting at €54.70 €65.09

Uranyless is a non-radioactive staining reagent as alternative to uranyl acetate. An optimized lanthanide Mix allows fast staining times. Uranyless is suitable for classical and negative staining procedures. The 30ml dispenser bottle is sufficient for staining of up to 1500 TEM grids.

UranyLess in 30% Ethanol is recommended for dense and lipid-rich tissue which is difficult to stain.
UranyLess in Acetone is used for contrasting cryo-fixed tissue during freeze substitution.

Recommended for use with Lead Citrate (#DM22410).

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Starting at €54.70 €65.09
Lead Citrate 3% - Ready to Use Solution

Starting at €54.70 €65.09

Lead Citrate is a commonly used metal stain for ultra-thin sections for electron microscopy. The Lead Citrate – Ready to Use Solution is ideal as counterstain for samples stained with UranyLess to enhance the contrast. The 3% solution is provided in a 30ml airless dispenser bottle and syringe.

Recommended for use with UranyLess (#DM22409).

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Starting at €54.70 €65.09

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We offer a large selection of chemicals for the preparation and processing of biological and material samples for light and electron microscopy. Our shop currently only shows a small part of it. You can find the entire range of our chemicals in the microscopy catalog.

Some chemicals e.g. toxic substances, radioactive substances are only available with permits or in compliance with the applicable legal requirements or only for designated countries.

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The safety data sheets can be found with the respective product or summarized in a table here.

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