Netwell™ Netwell Carrier Kit

Tissue Processing Well; Netwell™

A modular and disposable system which can be used for the following procedures:

• The handling and processing of tissue slices.
• In immunocytochemical staining.
• In 3-dimensional tissue culture.
• In explant tissue culture.

Home made devices are no longer necessary. These tissue processing wells are ready to fill all of your needs with the added convenience of being able to process up to 12 tissue specimens at one time (with one unit) .

Netwell™ Inserts
The Netwell™ is a polystyrene insert fitted with a polyester mesh bottom for use in 6 and 12-well plates. Available in three mesh sizes and two diameters; the Netwells provide ultimate flexibility in retaining fragile tissues during processing and staining.

Netwell™ Carrier Kit
A set of carrier plates and handles for moving the Netwells in and out of the 6 or 12-well plates. The handle can be attached or detached to the plate at any time.

To Load the Netwell Carriers
An easy method to load Netwells into the reusable carrier is to invert the plates leaving the netwells in the cover, then placing the carrier over the Netwells and reinverting the assembly.

To Use Handle:

1. Remove the plate lid
2. Grasp the handle, along its axis, between thumb and forefinger and squeeze (see drawing)
3. Insert both ends of the handle into carrier (see drawing) and release handle.
4. Change grip on handle 90° (this will prevent you from accidentally squeezing the handle and releasing the Netwells).
5. Pick up the Netwells and transport to the next work station.

Netwell™ Reagent Tray
Thermo-formed polystyrene. Holds 150 ml of reagent solution. Netwells are trans-ferred to the reagent tray by the Carrier kit, where fixing and staining is performed. Reagent trays are available in white for colorimetric reaction contrast, and in black for better visibility of tissue sections.

Applications
• The polyester mesh acts as a tissue carrier and strainer for immunocytochemical staining and biological and histological fixation. It avoids the risk of losing valuable samples associated with paint-brush transfer.
• For 3-D tissue culture, the sterile Netwell provides efficient media permeation and support to cultures grown in gels.
• As well, Sterile Netwells allow for easy processing of tissue explants and microcarrier-grown cells.