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HPF Carrier/Platelets

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High pressure freezing (HPF) carriers, also known as HPF planchettes or HPF plates, are sample holders that hold the sample in shape during high pressure freezing.

HPF carriers are essential components for sample preparation in electron microscopy. The carriers enable biological samples to be frozen quickly and evenly. Compared to chemical fixation structural artefacts are minimized during high pressure freezing. This is crucial for preserving the ultrastructural integrity of samples, making HPF a preferred method in biological and medical research.

HPF carriers are made from a variety of materials, with aluminum and sapphire being the most common.
Aluminum HPF carriers are characterized by their high thermal conductivity, which ensures rapid freezing of samples. These carriers are less expensive and are used for routine applications.
Sapphire HPF carriers, on the other hand, offer exceptional mechanical and chemical resistance. Sapphire is extremely hard and resistant to scratches and mechanical damage, making these carriers durable and ideal for demanding applications. In addition, sapphire is chemically inert and resists most chemical substances, making it suitable for use in aggressive environments. The optical transparency of sapphire is the main advantage as it allows samples to be observed before and after freezing in light microscopes (mostly fluorescence).

HPF carriers with vacuum-deposited or laser-engraved coordinate systems are available for localizing relevant regions of interest (ROI) in samples. These coordinates make it possible to find sample locations in the electron microscope that have previously been identified in the light microscope (CLEM - Correlative Light and Electron Microscopy).

 

    • €570.00 €678.30
      Art.Nr: W164450

      Achieve accurate correlation by imprinting a FinderTOP pattern in the sample surface during high pressure freezing (cryoCLEM). The pattern with unique marked squares allows precise targeting for cryoFIB/SEM volume imaging and cryoFIB lift out for cryoTEM.

      • Ø3mm x 0,50mm thick
      • 0,3mm cavity on one side
      • Grid with unique marked squares with side length 0,2mm (columns labeled A-L / rows labeled 1-12)
      €570.00 €678.30
      • Starting at €520.00 €618.80

        Sapphire version of the FinderTOP platelets. Imprinting a FinderTOP pattern into the sample surface during high-pressure freezing enables precise alignment for cryoFIB/SEM imaging and cryoFIB lift-out for cryoTEM. The transparent sapphire HPF carriers are particularly useful for correlating LM/EM images (cryoCLEM).

        • Ø3 x 0.16mm height
        • Grid with clearly marked squares with side length 0.2mm (A-L / 1-12)

        Starting at €520.00 €618.80

        • Starting at €104.00 €123.76

          High-Pressure-Freezing (HPF) specimen carriers made of aluminum with standard thickness suitable for the following high-pressure-freezers: HPM-010, Compact 01, 02, 03, HPM 100, Leica EM ICE, Leica EM PACT1 und 2.

          The carriers (planchets) are available in Type A, B and C. Packing unit 50, 100 or 200 pieces.

          Starting at €104.00 €123.76

          • Starting at €104.00 €123.76

            High-Pressure-Freezing (HPF) specimen carriers made of aluminium with optimized thickness for HPM100 and Leica EM ICE. The carriers (planchets) are available in Type A, B and C. Packing unit 50, 100 or 200 pieces.

            Starting at €104.00 €123.76

            • Starting at €5.00 €5.95

              High-Pressure-Freezing (HPF) specimen carriers made of aluminium suitable for the HPM 010 with 4,6mm diameter holder

              The carriers (planchets) are available in Type A, B and C.

              Starting at €5.00 €5.95