Technovit® 9100 Embedding Kit

Technovit® 9100 was specifically developed for the embedding of mineralised tissues as well as soft tissue with an expanded study spectrum in light microscopy. The deplasticized sections are suitable for histological overview staining, enzyme chemistry and immunohistological studies, including in-situ hybridisation. Thin sections for immunohistology can be stuck to glass object holders and deplasticized. Fields of application -Hard-cutting technique for making thin layers Examples: Iliac crest biopsies, smaller, spongy and compact bone tissue specimens -Division thin section technique (division procedure in point contact technology) Examples: Tooth/jaw areas with and without implants, non-cemented endoprostheses with shaft bones -Combined division-thin section technique and hard-cutting technique (target preparation) Examples: Boundary layer and environment assessment for metal implants and non-cemented endoprostheses Tissues that cannot be cut are teeth-bearing jaw sections with fillings, crowns and bridges, thick corticalis, implant-bearing (metal or ceramic) jaw or long bones, or brittle, hypermineralised bones. Material properties Polymerisation of the hydrophobic Technovit® 9100 occurs by excluding oxygen using a catalyst system made of peroxide and amine. Additional components such as PMMA powder and regulator allow for a controlled polymerisation in the cold (in the range of -2 to -20 °C, depending on the volume) that guarantees complete dissipation of the polymerisation heat. The benefits of the system at a glance -Polymerisation below freezing -Reproducibility of the embedding results and reliability due to constant, documented quality controls -Equal block hardening -The PMMA block remains transparent -Better results with regard to cutting and staining because Technovit® 9100 contains a hydrophilising agent -Can be used for thin section and the sawing and cutting techniques -Enzyme histology and immunohistology as well as in-situ hybridisation possible (sections)