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Thiocarbohydrazide (TCH), Highest Purity, 5g

E21900

Osmiophilic reagent. With the Thiocarbohydrazide it´s possible to enhance the contrast of all osmophilic components of the cell, especially lipids, which hold the most osmium.

An indefinite shelf life due to a special purification process.

H2NNHCSNHNH2 / m.p. 171°C (decomposed)

 

 

€223.80 €266.32

Product Details

Description

Thiocarbohydrazide, Highest Purity, (TCH)

  •     99% minimum purity - NH 2 NHCSNHNH 2
  •     Pure white crystalline form M.P. is 172°C
  •     Indefinite shelf-life due to purification procedure
  •     Does not need to be kept refrigerated


Staining method:

(OTO) This staining method (osmium fixed tissue exposed to TCH followed by a second treatment with osmium) enhances the contrast of all osmophilic components of the cell, especially lipids, which hold the most osmium.

Osmium tetroxide fixed tissue is embedded in Araldite, sectioned (ultrathin), and placed on nickel, or gold grids, without a supporting membrane. They are then exposed to a 1% hot aqueous solution of TCH for 1 hour at 50°C, followed by several washes with hot water (50°C) for 10 to 15 minutes to remove any unbound TCH. The sections are then exposed to OsO4. There are two methods for the second OsO4 exposure: (1) exposure to OsO4 vapor for 1 hour in a closed vial suspended in a 60°C water bath or, (2) treating the grids with a 2% solution of OsO4 (unbuffered) at 50°C for 1 hour followed by a brief wash.

Reference:

  • Seligman A.M. et al (1965). Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH) or thiosemicarbazide (TSC) and osmium tetroxide. J. Histochem. Cytochem., 13:629
  • Seligman, A.M. et al (1966). A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide fixed tissue with osmiophilic thiocarbohydrazide (TCH). J. Cell Biol., 30:424
  •  Thiery, J.P. (1967). Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J. Microsc., 6:987
  • Lo, H.K. et al (1987). A modified periodic acid-thiocarbo-hydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. J. Histochem. Cytochem., 35:393
  • Neiss, W.F. (1988). Enhancement of the periodic acid-Schiff (PAS) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) reaction in LR White sections. Histochemistry, 88:603
  • Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science, N.Y., 146:1039
  •  Derenzini, M., et al (1986). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glyco-conjugates at the molecular level in situ. J. Histochem. Cytochem.,34:1161

More Information

More Information
Symbol GHS
GHS05 GHS05
GHS06 GHS06
GHS07 GHS07
Signal word
Danger
Mandatory sign
Tightly sealed goggles Tightly sealed goggles
Protective gloves Protective gloves
Hazard statements
H301
H312_H332
H318
Precautionary statements
P321
P330
P362
P301_310
P305_351_338
P405
P501
Application
EM
Storage Temperature
RT
Shipping Class
Hazardous Good LQ
Packing Unit 5g
SDS (multilingual) E21900