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CitiFluor™ AF1 plus DAPI, 15ml

E17970-125

It is a non-hardening antifading/anti-bleaching mounting medium for fluorescent samples containing 2µg/ml DAPI. Citifluor AF1 plus DAPI is a glycerol solution buffered with PBS containing a amine-based antifadent. The mounting medium is ideally suited for staining of nuclei for dead/fixed cells, immunolabeling with fluorescent antibodies and FISH.

Refractive index at 20°C: 1.463; pH: ~10; optically transparent from 300 to 750nm.

€75.30 €89.61

Product Details

Description

Description

This is a new addition to the range of Citifluor products and will be of value to those using the FISH technique.

Properties and Storage of AF1 plus DAPI mountant solution

The solution is of medium viscosity and has a water-white appearance. It is recommended that the solution is kept in a tightly sealed brown glass bottle and stored at 5°-15°C or in a refrigerator at a temperature between 0° and 5°C and out of strong sunlight. The cap of the bottle (or if using the pipette supplied with the material, the cap which covers the pipette delivery point) should always be replaced after use since the solution is hygroscopic. Samples stored under these conditions for 6 months have shown no apparent deterioration.

 

Usage and Useful Tips

If a lower concentration of DAPI is required, dilution should be carried out by adding a further quantity of AF1 solution. The solution will be of value to those using the FISH technique.

The solution should be pipetted onto the specimen and then a cover slip applied. If the slides are stored in a refrigerator, the viscosity of the mountant solution increases thereby helping too keep the cover slip in place. There is no need to seal the cover slip with nail varnish.

 

For more information please see our Info-Box.

More Information

More Information
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Packing Unit 15ml
ready to use yes
Shipping Class
Standard
Used for Fluorescence
yes
Manufacturer
EMS
SDS (multilingual) E17970-125