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EVE™ HT FL Automated High-Throughput Fluorescence Cell Counter


The EVE™ HT FL is a high-throughput automated fluorescence cell counter equipped with bright field and two fluorescence channels, UV for DAPI and blue for Acridin Orange (AO) detection. In only 3 minutes, up to 48 samples can be counted and analyzed. EVE™ HT FL delivers highest precision and accuracy for both cell lines and primary cell analysis in a variety of applications.

Product Details


Key Features and Benefits

  • Dual-Fluorescence (AO/DAPI) & Bright Field 
  • Fast counting: 48 Samples in 3 min
  • Only 20 μL sample volume required
  • Measures Real Cell Size & Viability
  • Accurately identify and count agglomerated cells



Staining dye(s) AO/DAPI mixed solution
Measuring range Detectable range: 1 x 10E4 ~ 2 x 10E7 cells/mL
Optimal range: 1 x 10E5 ~ 1 x 10E7 cells/mL
Cell size range Detectable size: 5 ~ 85 μm
Optimal size: 5 ~ 80 μm
Analysis time 3 ~ 18 minutes / 48 tests
Loading volume 20 μL / channel
Light sources Bright field, UV, Blue LED
Dimensions 586 (W) x 461 (D) x 458 (H) mm


61 kg



Dual-Florescence for Accurate Viability Analysis of Primary Cells 

AO (Acridine Orange) and DAPI (4′, 6-diamidino-2-phenylindole) are fluorescent dyes that stain the nucleus of targeted cells by intercalation into the DNA.

AO is a permeable dye that stains the nucleus of all cells, regardless of the cell’s condition. DAPI is a dye with limited permeability that stains the nucleus of non-viable cells with damaged membranes very well.

To accurately evaluate the viability of cell types like PBMCs (Peripheral Blood Mononuclear Cell), stem cells, and splenocytes, it is advisable to use dual-fluorescence staining. 

This method effectively excludes non-nucleated cells (e.g. Erythrocytes or platelets) and debris from the samples.


AO/DAPI staining - Counting & Viability


Accurate Fluorescent Cell Counting for PBMCs

PBMCs are often mixed with red blood cells (RBCs) or platelets. Use EVE™ HT FL to count only nucleated cells such as PBMCs. Fluorescence based counting is more accurate than traditional Trypan Blue based counting.


Up to 48 Samples in 3 minutes

Count your samples in just 3 minutes. 
Leading to substantial time saving by eliminating the need for frequent reloading and waiting periods.


20 μL of sample volume

EVE™ HT FL only needs 20 μL of your valuable samples. Save your cells for more measurements or better outcome.


Level Up Your Productivity

Level up your productivity with EVE™ HT FL compared to other cell counters.


Reliable, Accurate and Precise Results

  • Multi-Instrument Consistency & Precision for Cells
    EVE™ HT FL instruments were tested using three different cell line samples (Jurkat, HeLa, and CHO) to compare their differences.

    The result below showed a high instrument-to-instrument consistency with small variation.

  • Excellent Linearity Across Wide Range
    Following ISO standard for cell counting, we evaluated linearity of EVE™ HT FL using 2 cell lines (CHO cells and Jurkat cells). The following results demonstrate highest linearity.

  • High correlation between EVE™ HT FL and flow cytometer
    Cell samples were measured using EVE™ HT FL and a flow cytometer.
    For both CHO cells and PBMCs, total cell counts measured by EVE™ HT FL were highly correlated with those measured by a flow cytometer.


FDA 21 CFR Part 11 Compliance READY*

EVE™ HT FL is ready for 21 CFR Part 11 compliance for cGMP facilities.


*(Code of Federal Regulations, which sets forth the United States Food and Drug Administration's (FDA) regulations on electronic records and electronic signatures (ERES). Part 11 defines the criteria by which electronic records and electronic signatures are considered trustworthy, reliable, and equivalent to paper records.)


User-friendly Graphical User Interface (GUI)

With visualized data, the results are clear at a glance.

(Total cell, Live/Dead Cell, Viability, Cell Size)


More Information

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