Prepmaster 5100, TEM Sample Preparation Robot and Accessories

The Prepmaster 5100 is a fully automated system for the preparation of multiple biological samples for TEM. The advanced robotics and precise liquid handling guarantee reliable, consistent and fully automated execution of specimen preparation protocols. Ideal for core facilities or laboratories with high sample throughput or complex preparation protocols.

Grouped product items
Product Name	Qty
Prepmaster 5100, TEM Sample Preparation Robot System E51000
96-well Conical Plate for Specimen for Prepmaster 5100, 200µl, 20 pieces E51000-10
96-well Reagent Reservoir for Prepmaster 5100, square, 1,2ml, 10 pieces E51000-20
Bulk Reagent Reservoirs for Prepmaster 5100, 48 pieces E51000-25
Pipette Tips for Prepmaster 5100 in 96-well Box, 300µl, 100 boxes E51000-30

Product Details

Description

The Prepmaster 5100 Specimen Preparation Robot System

Features

Heated Agitation Station™ specimen dock with gentle, constant shaking movement for rapid and thorough post-fixation and rinsing (RT–60°C).
Heated reagent reservoir for enhanced post-fixation with hot heavy metals or other reagents (RT–60°C).
Cooled reagent reservoir for cold dehydration or cold reduced osmium (5°C–RT).
Windows® laptop computer control for easy creation, modification, and storage of unlimited protocols.
UV light protected ventilated enclosure keeps noxious fumes contained and vented.
Small footprint (63 x 57cm) enables convenient in-hood option with enclosure removed.

Benefits

Error reduction due to eliminating pipetting errors or mishandling
Easy to set up and clean
Versatile - processing of most biological samples
Fast Operation - e.g. Prepare up to 8 kidney samples in less than 1 hour
Excellent for complicated protocols e.g. volume EM sample preps (ROTO protocols)
Can handle up to 24 unique reagents or rinses.
Unattended operation overnight
Reproducible, high quality and consistent results

Specifications

Dimensions (W x D x H)	63 x 57 x 66 cm (25 x 22.5 x 26 in.)
Weight	48kg (105.8 lb.)
Frame Composition	Rigid steel and CNC aluminum
Operating Environment	Temperature: Recommended 20-24 °C Relative Humidity: Recommended 40-60%
Power Requirements	Robot Power Input: 36 VDC, 6.1 A Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
Minimum Operating System Requirements	1.7 GHZ processor, 4GB Memory, 256 GB SSD and Win 10 Pro.
Connectivity	WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
Certifications	CE, FCC, NRTL, CB, ISO 9001
Pipette Configurations	8-channel 300 μl pipetting head
Pipette Volumes	8-channel: 1-300 μl

Precision meets efficiency with 1-2-3 ease

1. Load Reagents
Add sealed reservoir with freshly prepared osmium tetroxide and other hazardous reagents.
Buffer, water, and ethanol are stored in bulk reservoirs and refilled daily.

2. Load Specimen
Transfer biological specimens to the sample processing plate. Set plate on sample dock.

3. Select Protocol & Press Start
Select your preprogrammed protocol and press Start.
Then walk away. The Prepmaster will alert you when it’s finished running.

Applications

The versatile Prepmaster 5100 offers a wide range of applications for TEM, SEM, and LM including, but not limited to, tissue/biopsies (1mm dia. x 1–3mm length), cells seeded-on-coverslips, organoids, retinas, and most other biological samples.


Mouse Ovaries Ru-ching Hsia, Carnegie Institute of Science	Plant Root Ru-ching Hsia, Carnegie Institute of Science Hypocotyl taproot of Aeschynomene americana, a plant of the legume family commonly known as shy leaf or American joint vetch.	Liver Biopsy Ellisman OTO protocol	Cardiac Muscle Ben August, UW-Madison, Ellisman rOTO Protocol

Astrocytes on Coverslips

Ben August, UW-Madison
Cacodylate buffer with reduced osmium tetroxide shows beautifully preserved Golgi apparatus membranes, low cytoplasmic background, and easily visible microtubules.

The Prepmaster 5100 Specimen Preparation Robot System includes:

Robot:

Prepmaster 5100 Specimen Preparation Robot chassis
Ventilated 99% UV blocking fume containment and evacuation enclosure
Thermally Controlled (RT–60°C) Agitation Station™ Sample Dock
Heated (RT - 60°C) 12-position Reagent Reservoir
Cooled (5°C - RT) 12-position Reagent Reservoir
Windows laptop computer controller with power supply
USB cable

Labware:

1x 4-position bulk reagent reservoir with 1 set of 4 reusable reservoirs
1x 96-well reservoir for heavy metals and other toxic reagents
1x 96-well reservoir for toxic reagent waste
1x 96-well reservoir for dehydration reagents
1x 1-well reservoir for non-toxic waste
1x Squeeze bottle for phosphate buffer refilling
1x Squeeze bottle for ethanol refilling
1x Squeeze bottle for water refilling

Consumables:

2x boxes of 96-well 300μl pipette tips
10x specimen prep plates
10x 1ml graduated transfer pipettes
10x X-pierce vinyl plate covers
10x aluminum plate seals

More Information

More Information
Dimensions	63x57x66 cm (25 22.5x26 in.)
Electrical Power	Robot Power Input: 36 VDC, 6.1 A Power Adapter Input: 100-240 VAC, 50/60 Hz, 4.0 A/115 VAC, 2.0 A/230 VAC
Weight	48kg (105.8 lb.)