The Triax™ Flow Cell from BioComp is a new developed and patented detector for the Piston Gradient Fractionator™. So far just UV absorption spectra recording was possible during fractionation.
The new Triax™ Flow Cell provides the ability to record UV adsorption as well as VIS fluorescence scans. The scans can be obtained simultaneously in the same gradient, offering best precision in the overlay. Depending on the configuration, it is possible to scan simultaneously up to three wavelengths.
The Triax™ Flow Cell offers numerous possibilities for your application.
Three options of the Triax™ Flow Cell are available:
FC-1 is the single wavelength version. It is intended to replace the Bio-Rad EM1, but can also be configured to record fluorescence, allowing overlaying of separate VIS and UV scans. Wavelength and fluorophore changes are made by changing the LEDs, photodiodes and filters.
FC-2 allows two input wavelengths, so the UV and VIS scans are obtained in the same gradient. Another possible use is the dual UV scan at 260 and 280 nm, providing a 260/280 ratio that reflects the change in nucleotide/protein composition of a particle.
FC-3 is used for three wavelength scans, one UV scan for the UV profile and the other one or two VIS scans for fluorescence profiles in their particle’s peaks. Up to three wavelength scans can be recorded simultaneously in the same gradient.
All LEDs are driven simultaneously by using the newest Triax™ software.